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hindiii restriction sites  (TaKaRa)


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    Structured Review

    TaKaRa hindiii restriction sites
    Hindiii Restriction Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hindiii+restriction+sites/Hind+III/pm42103020-62-16-19
    Average 99 stars, based on 9144 article reviews
    hindiii restriction sites - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cloning:

    Article Title: Biologically-targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett's esophagus and esophageal adenocarcinoma.
    Article Snippet: Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, Puya Gharahkhani, Xiang Shu, Kenneth Offit, Pari M. Shah, Hans Gerdes, Daniela Molena, Amitabh Srivastava, Stuart MacGregor, BEACON Consortium, OCCAMS Consortium, Esophageal Cancer Consortium, Claire Palles, René Thieme, Michael Vieth, Ines Gockel, Thomas L. Vaughan, Johannes Schumacher, Matthew F. Buas

    Article Title: KLF4 regulates skeletal muscle development and regeneration by directly targeting P57 and Myomixer.
    Article Snippet: Vector construction and dual luciferase reporter assay The promoter sequences of the mouse P57 and Myomixer genes, including predicted KLF4 binding motifs, were obtained from the NCBI database (www.ncbi.nlm.nih.gov) and amplified by PCR, with the KLF4 binding motif identified by ChIP-qPCR being mutated. .. The wild-type and mutated promoter were respectively inserted into the pGL3.0-basic vector between the KpnI and HindIII restriction sites using the In-Fusion HD Cloning kit (Takara Bio). .. When the density reached 70%, C2C12 cells were transfected with 500 ng of the wild-type or mutated promoter construct mixed with 50 ng of Renilla luciferase vector (pRL-TK) using Lipofectamine 3000 (Invitrogen).

    Article Title: rBCG::PUMA exhibits enhanced immunogenicity compared to BCG in mice.
    Article Snippet: Background: Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains a major global public health threat.. The limited efficacy of the current Bacillus Calmette–Guérin (BCG) vaccine against adult pulmonary TB highlights the urgent need for improved vaccine strategies.. To address this, we engineered a recombinant BCG strain, designated rBCG::PUMA, which expresses the pro-apoptotic gene PUMA to enhance immunogenicity.

    Variant Assay:

    Article Title: Biologically-targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett's esophagus and esophageal adenocarcinoma.
    Article Snippet: Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, Puya Gharahkhani, Xiang Shu, Kenneth Offit, Pari M. Shah, Hans Gerdes, Daniela Molena, Amitabh Srivastava, Stuart MacGregor, BEACON Consortium, OCCAMS Consortium, Esophageal Cancer Consortium, Claire Palles, René Thieme, Michael Vieth, Ines Gockel, Thomas L. Vaughan, Johannes Schumacher, Matthew F. Buas

    Article Title: Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinoma
    Article Snippet: .. DNA fragments containing alternate alleles of the prioritized risk variant rs4841556 were synthesized by Twist Biosciences and cloned into the minimal-promoter-NanoLuc reporter vector pNL3.1 (Promega, no. N1031) between KpnI and HindIII restriction sites in both orientations using In-fusion Snap Assembly Master Mix (Takara, no. 638947). ..

    Synthesized:

    Article Title: Biologically-targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett's esophagus and esophageal adenocarcinoma.
    Article Snippet: Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, Puya Gharahkhani, Xiang Shu, Kenneth Offit, Pari M. Shah, Hans Gerdes, Daniela Molena, Amitabh Srivastava, Stuart MacGregor, BEACON Consortium, OCCAMS Consortium, Esophageal Cancer Consortium, Claire Palles, René Thieme, Michael Vieth, Ines Gockel, Thomas L. Vaughan, Johannes Schumacher, Matthew F. Buas

    Article Title: Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinoma
    Article Snippet: .. DNA fragments containing alternate alleles of the prioritized risk variant rs4841556 were synthesized by Twist Biosciences and cloned into the minimal-promoter-NanoLuc reporter vector pNL3.1 (Promega, no. N1031) between KpnI and HindIII restriction sites in both orientations using In-fusion Snap Assembly Master Mix (Takara, no. 638947). ..

    Clone Assay:

    Article Title: Biologically-targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett's esophagus and esophageal adenocarcinoma.
    Article Snippet: Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, Puya Gharahkhani, Xiang Shu, Kenneth Offit, Pari M. Shah, Hans Gerdes, Daniela Molena, Amitabh Srivastava, Stuart MacGregor, BEACON Consortium, OCCAMS Consortium, Esophageal Cancer Consortium, Claire Palles, René Thieme, Michael Vieth, Ines Gockel, Thomas L. Vaughan, Johannes Schumacher, Matthew F. Buas

    Article Title: Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinoma
    Article Snippet: .. DNA fragments containing alternate alleles of the prioritized risk variant rs4841556 were synthesized by Twist Biosciences and cloned into the minimal-promoter-NanoLuc reporter vector pNL3.1 (Promega, no. N1031) between KpnI and HindIII restriction sites in both orientations using In-fusion Snap Assembly Master Mix (Takara, no. 638947). ..

    Construct:

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a‐FvBgls3 138‐580 . ..

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity.
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a- FvBgls3138- 580. ..

    Homologous Recombination:

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a‐FvBgls3 138‐580 . ..

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity.
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a- FvBgls3138- 580. ..

    Recombinant:

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a‐FvBgls3 138‐580 . ..

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity.
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a- FvBgls3138- 580. ..

    Plasmid Preparation:

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a‐FvBgls3 138‐580 . ..

    Article Title: Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinoma
    Article Snippet: .. DNA fragments containing alternate alleles of the prioritized risk variant rs4841556 were synthesized by Twist Biosciences and cloned into the minimal-promoter-NanoLuc reporter vector pNL3.1 (Promega, no. N1031) between KpnI and HindIII restriction sites in both orientations using In-fusion Snap Assembly Master Mix (Takara, no. 638947). ..

    Article Title: A Coumarin Compound Derived From Zanthoxylum avicennae Reduces the Pathogenicity of Fusarium verticillioides by Directly Binding to and Inhibiting Glycoside Hydrolase 3 Activity.
    Article Snippet: .. EcoRI and HindIII restriction sites were selected, and the fragment was constructed into the vector pET28a (+) using homologous recombination enzyme (TaKaRa), yielding the recombinant plasmid pET28a- FvBgls3138- 580. ..

    Article Title: Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy.
    Article Snippet: .. Sequence coding for GFP was PCR-amplified using primers incorporating BamHI and HindIII restriction sites (Supplementary Table S2) and plasmid pGFP-N3 (Clontech, Takara Bio Inc., Japan) as a template. ..

    Article Title: KLF4 regulates skeletal muscle development and regeneration by directly targeting P57 and Myomixer.
    Article Snippet: Vector construction and dual luciferase reporter assay The promoter sequences of the mouse P57 and Myomixer genes, including predicted KLF4 binding motifs, were obtained from the NCBI database (www.ncbi.nlm.nih.gov) and amplified by PCR, with the KLF4 binding motif identified by ChIP-qPCR being mutated. .. The wild-type and mutated promoter were respectively inserted into the pGL3.0-basic vector between the KpnI and HindIII restriction sites using the In-Fusion HD Cloning kit (Takara Bio). .. When the density reached 70%, C2C12 cells were transfected with 500 ng of the wild-type or mutated promoter construct mixed with 50 ng of Renilla luciferase vector (pRL-TK) using Lipofectamine 3000 (Invitrogen).

    Article Title: Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy
    Article Snippet: .. Sequence coding for GFP was PCR-amplified using primers incorporating BamHI and HindIII restriction sites ( ) and plasmid pGFP-N3 (Clontech, Takara Bio Inc., Japan) as a template. ..

    Article Title: rBCG::PUMA exhibits enhanced immunogenicity compared to BCG in mice.
    Article Snippet: Background: Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains a major global public health threat.. The limited efficacy of the current Bacillus Calmette–Guérin (BCG) vaccine against adult pulmonary TB highlights the urgent need for improved vaccine strategies.. To address this, we engineered a recombinant BCG strain, designated rBCG::PUMA, which expresses the pro-apoptotic gene PUMA to enhance immunogenicity.

    Sequencing:

    Article Title: Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy.
    Article Snippet: .. Sequence coding for GFP was PCR-amplified using primers incorporating BamHI and HindIII restriction sites (Supplementary Table S2) and plasmid pGFP-N3 (Clontech, Takara Bio Inc., Japan) as a template. ..

    Article Title: Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy
    Article Snippet: .. Sequence coding for GFP was PCR-amplified using primers incorporating BamHI and HindIII restriction sites ( ) and plasmid pGFP-N3 (Clontech, Takara Bio Inc., Japan) as a template. ..

    Polymerase Chain Reaction:

    Article Title: Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy.
    Article Snippet: .. Sequence coding for GFP was PCR-amplified using primers incorporating BamHI and HindIII restriction sites (Supplementary Table S2) and plasmid pGFP-N3 (Clontech, Takara Bio Inc., Japan) as a template. ..

    Article Title: Protein trans-splicing: optimization of intein-mediated GFP assembly as a model for the development of gene therapy
    Article Snippet: .. Sequence coding for GFP was PCR-amplified using primers incorporating BamHI and HindIII restriction sites ( ) and plasmid pGFP-N3 (Clontech, Takara Bio Inc., Japan) as a template. ..



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